BPC-157/TB-500
Regenerative peptide combination
BPC-157 primarily influences localized tissue repair signaling, vascular integrity, nitric oxide pathways, and fibroblast migration. TB-500 is associated with systemic actin regulation, cellular migration, wound remodeling, and regenerative signaling cascades.
$74.00
In stock
Guaranteed Safe Checkout
Research Data
Tissue Repair Capacity Decline
Regenerative pathway activity vs. age (% of peak)
Literature
Cellular Ratio
Biological Intersections
Relative pathway engagement
Activity Profile
BPC-157 / TB-500 Repair Profile
Mechanism
Cellular Pathway
01
BPC-157: VEGF Upregulation
BPC-157 stimulates VEGF expression and promotes neovascularization, supplying injured tissue with oxygen and nutrients essential for repair.
02
TB-500: Actin Sequestration
TB-500 (Tβ4 fragment) sequesters G-actin and regulates cytoskeletal dynamics, promoting cell migration into wound sites and accelerating tissue remodeling.
03
Synergistic GIT/CNS Repair
Combined BPC-157 and TB-500 activity modulates growth hormone receptor signaling and promotes healing in gastrointestinal, musculoskeletal, and neural tissue models.
Metabolic Network
Biosynthesis Map
Sequence Analysis
Amino Acid Sequences
Single-letter residue map colored by physicochemical property class. Hover any residue for full name and position.
BPC-157
G
E
P
P
P
G
K
P
A
D
D
A
G
L
V
TB-500
L
K
K
T
E
T
Q
■ Hydrophobic
■ Polar
■ Positively Charged
■ Negatively Charged
■ Glycine
Research Focus
Regenerative Pathways
Sequential activation strength
Product Data
Compound Identity
| Product Name | BPC-157 / TB-500 Blend |
| Functional Class | Tissue Repair & Angiogenesis |
| Form | Lyophilized |
| Purity | ≥98% |
| Content | 5mg |
| Count | 1 vial |
| Research Use | For in vitro and laboratory research use only. Not for human consumption. |
Specifications
Technical Specs
| CAS Number | 137525-51-0 / 77591-33-4 |
| Molecular Weight | BPC-157: ~1419.5 g/mol / TB-500: ~4963.5 g/mol |
| Molecular Formula | BPC-157: C62H98N16O22 / TB-500: C212H350N56O78S |
| PubChem CID | See COA |
| Appearance | White lyophilized powder |
| Storage | –20°C long-term / 2–8°C short-term |
Formulation Reference
Anatomy of a Peptide
A reference guide to the components of a lyophilized research peptide — from the active sequence to the excipients, solvents, buffers, and stabilizers used in formulation.
Active Peptide 2 items
Synthetic Amino Acid Sequence
The primary chain of amino acids synthesized via solid-phase peptide synthesis (SPPS). Defined by sequence length and molecular weight.
Peptide Modifications
Acetylation (N-terminus), amidation (C-terminus), PEGylation, or cyclization applied to improve stability, receptor binding, or half-life.
Excipients 4 items
Mannitol
Sugar alcohol bulking agent that forms an elegant lyophilized cake, aids reconstitution, and provides structural matrix during freeze-drying.
Trehalose
Non-reducing disaccharide that stabilizes peptide secondary structure by replacing water molecules through hydrogen bonding during dehydration.
Sucrose
Disaccharide used as a lyoprotectant and tonicity agent. Forms an amorphous glassy matrix that immobilizes the peptide and prevents aggregation.
Glycine
Amino acid bulking agent used in lyophilization. Crystallizes to provide mechanical strength to the freeze-dried cake structure.
Reconstitution Solvents 4 items
Bacteriostatic Water (BAC Water)
Sterile water containing 0.9% benzyl alcohol as a preservative. Preferred for multi-dose vials — inhibits microbial growth after initial puncture.
Sterile Water for Injection
USP-grade water, pyrogen-free, without preservatives. Used for single-dose preparations or when benzyl alcohol sensitivity is a concern.
Acetic Acid Solution (0.1–1%)
Dilute acid used for peptides with poor aqueous solubility at neutral pH. Protonates basic residues to improve dissolution.
Sodium Chloride 0.9%
Isotonic saline diluent. Provides physiological osmolality (~308 mOsm/L) and can improve stability of certain charged peptides.
Buffer Systems 4 items
Phosphate Buffered Saline (PBS)
Maintains pH 7.2–7.4. Composed of sodium phosphate dibasic, potassium phosphate monobasic, NaCl, and KCl. Mimics physiological ionic strength.
Acetate Buffer
Effective pH range 3.7–5.6. Composed of acetic acid and sodium acetate. Ideal for acidic peptides and those requiring lower pH for solubility.
Citrate Buffer
Effective pH range 3.0–6.2. Offers strong buffering capacity and metal-chelating properties. Used when oxidation-sensitive residues (Met, Cys) are present.
Histidine Buffer
Effective pH range 5.5–7.0. Low ionic strength, minimal interaction with peptides. Increasingly preferred in modern biopharmaceutical formulations.
Lyoprotectants & Cryoprotectants 3 items
Trehalose / Sucrose (Lyoprotectant)
Protect peptide conformation during the drying phase of lyophilization by forming hydrogen bonds that substitute for water molecules around the peptide.
Glycerol (Cryoprotectant)
Polyol that depresses the freezing point and reduces ice crystal formation, preventing mechanical damage to peptide structure during freezing steps.
Polyethylene Glycol (PEG)
Hydrophilic polymer that provides steric stabilization, reduces aggregation, and can serve as both cryoprotectant and solubility enhancer.
Preservatives & Antimicrobials 3 items
Benzyl Alcohol (0.9%)
Aromatic alcohol preservative in bacteriostatic water. Acts as antimicrobial agent by disrupting microbial cell membranes. Standard for multi-use vials.
Methyl / Propyl Parabens
Broad-spectrum antimicrobial preservatives effective against fungi and bacteria. Used in some peptide formulations where benzyl alcohol is incompatible.
Phenol (0.5%)
Bacteriostatic preservative used in certain injectable peptide formulations. Also acts as a conformational stabilizer for some peptide structures.
Counter Ions & Salt Forms 3 items
Trifluoroacetate (TFA)
Most common counter ion from RP-HPLC purification. Forms TFA salt with basic residues (Lys, Arg, His). May affect bioassay results and cell toxicity.
Acetate
Milder alternative to TFA obtained via ion exchange. Lower cytotoxicity, preferred for cell-based research assays and in vivo studies.
Hydrochloride (HCl)
Chloride salt form, sometimes used for improved stability or specific solubility profiles. Common in pharmaceutical-grade peptide preparations.
Chelating Agents 2 items
EDTA (Disodium)
Chelates divalent metal ions (Cu²⁺, Fe²⁺, Zn²⁺) that catalyze oxidative degradation of methionine and cysteine residues in peptides.
Citric Acid
Natural chelator with moderate metal-binding capacity. Dual function as buffer component and oxidation inhibitor in peptide formulations.
Antioxidants & Stabilizers 3 items
L-Methionine
Free methionine added as a sacrificial antioxidant. Preferentially oxidizes before methionine residues within the peptide chain.
Ascorbic Acid
Water-soluble antioxidant that scavenges reactive oxygen species. Used at low concentrations to prevent oxidative peptide degradation.
Polysorbate 20 / 80
Non-ionic surfactants that prevent surface adsorption and aggregation of peptides at air-liquid and container-liquid interfaces.
Preparation Tool
Reconstitution Calculator
Enter your target working concentration to calculate the exact solvent volume needed for this vial.
mg
Solvent Volume Required
—
Recommended solvents
Bacteriostatic Water
Sterile Water for Injection
Acetic Acid 0.1%
Sodium Chloride 0.9%
Product Specs
Solubility Profile
| Water | Highly soluble |
| Acidified Water | Highly soluble |
| DMSO | Highly soluble |
| Ethanol | Moderate |
| Lipid solvents | Poor compatibility |
Product Specs
Storage Specs
| Lyophilized | 2–8°C preferred |
| Long-term | −20°C recommended |
| Light Sensitivity | Moderate |
| Moisture | High sensitivity |
| Stability | Stable when dry |
| Container | Sterile sealed vial |
Literature
Research Citations
Catalogue Pathway
Related Systems
Use this for internal linking, adjacent products, and quick route-back buttons.
Important Notice
Research Use Only
AminoBox products are supplied for research, analytical, and laboratory use only. Product information is provided for educational and technical reference and does not constitute medical advice. Products are not intended to diagnose, treat, cure, or prevent any disease.
About This Data
Chart Explanation
What this chart is about
Research context
Research Outcome
Blend Composition
| Compound | Content |
|---|---|
| BPC-157 | 5mg |
| TB-500 (Thymosin Beta-4 Fragment) | 5mg |
| Total Peptide Content | 10mg |
Additional Scientific Details
| Category | BPC-157 | TB-500 (Thymosin Beta-4 Fragment Analog) |
|---|---|---|
| Full Name | Body Protection Compound-157 | Thymosin Beta-4 Fragment Analog |
| CAS Number | 137525-51-0 | No universally standardized CAS for “TB-500” specifically |
| Molecular Formula | C62H98N16O22 | Varies depending on peptide fragment/manufacturer |
| Molecular Weight | ~1419.56 g/mol | ~4963 g/mol (full Thymosin Beta-4 reference) |
| Peptide Chain | Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val | Synthetic fragment analog derived from Thymosin Beta-4 |
| Primary Research Category | Angiogenic & tissue repair peptide | Actin-regulating regenerative peptide |
| Primary Mechanisms | VEGF modulation, NO signaling, fibroblast migration | Actin binding, cellular migration, wound remodeling |
| PubMed Reference ID | PMID: 21030672 | PMID: 25950542 |
| Common Synonyms | BPC-157, Pentadecapeptide BPC-157, PL-14736 | TB-500, Thymosin Beta-4, Tβ4 |
Compound Overview
| Property | Specification |
|---|---|
| Product Type | Dual-peptide research blend |
| Peptide Class | Synthetic regenerative peptides |
| Appearance | White lyophilized powder |
| Purity | Typically ≥98% |
| Formulation Type | Lyophilized peptide blend |
| Reconstitution | Bacteriostatic water compatible |
Peptide 1 — BPC-157 Specifications
| Property | Specification |
|---|---|
| Chemical Name | Body Protection Compound-157 |
| Molecular Formula | C62H98N16O22 |
| Molecular Weight | 1419.53 g/mol |
| Amino Acid Sequence | GEPPPGKPADDAGLV |
| Peptide Length | 15 amino acids |
| Compound Type | Synthetic gastric peptide fragment |
| Solubility | Water soluble |
| Research Focus | Tissue signaling and recovery pathways |
Structural Classification
| Category | Description |
|---|---|
| Compound Type | Multi-peptide regenerative blend |
| Functional Class | Tissue recovery and signaling research |
| Biological Focus | Cellular repair and recovery pathways |
| Mechanistic Focus | Angiogenesis, migration, and tissue remodeling research |
Research Areas Commonly Associated
(avoid framing as medical claims)
- Recovery signaling research
- Tissue remodeling pathways
- Musculoskeletal research
- Angiogenesis research
- Connective tissue studies
- Cellular migration pathways
- Soft tissue recovery research
Solubility Profile
| Solvent | Solubility |
|---|---|
| Bacteriostatic Water | Highly soluble |
| Sterile Water | Soluble |
| Acetic Acid Solutions | Compatible |
| DMSO | Limited use |
Storage Specifications
| Parameter | Recommendation |
|---|---|
| Lyophilized Storage | -20°C preferred |
| Refrigerated Storage | 2–8°C |
| Reconstituted Stability | Limited; refrigerated use recommended |
| Light Sensitivity | Moderate |
| Moisture Sensitivity | High |
| Container Type | Sterile amber vial |
Technical Characteristics
| Feature | Notes |
|---|---|
| Delivery Format | Lyophilized injectable peptide blend |
| Compound Stability | Stable under frozen dry storage |
| Reconstitution | Bacteriostatic water recommended |
| Research Use | Laboratory research only |
BPC-157 + TB-500 is a dual-peptide research formulation combining two extensively studied regenerative peptides investigated for their roles in tissue repair, angiogenesis, cytoskeletal remodeling, and cellular recovery signaling. Together, these peptides are studied as a multi-pathway regenerative system targeting recovery dynamics across connective tissue, muscle, tendon, ligament, vascular, and epithelial structures.
BPC-157 Overview (Body Protection Compound-157)
BPC-157 is a synthetic pentadecapeptide derived from a protective protein sequence originally isolated from gastric juice.
In research literature, BPC-157 is studied for its influence on:
- Tissue regeneration signaling
- Angiogenic pathway modulation
- Nitric oxide system regulation
- Fibroblast migration and collagen organization
- Gastrointestinal epithelial integrity
- Tendon-to-bone healing dynamics
Unlike many peptides that act through highly specific receptor systems, BPC-157 appears to influence broad cytoprotective and regenerative signaling networks.
BPC-157 Mechanistic Research
1. Angiogenesis & Vascular Repair
BPC-157 is strongly associated with modulation of:
- VEGF (vascular endothelial growth factor) pathways
- Endothelial cell migration
- Microvascular circulation and capillary formation
This may support:
- Oxygen and nutrient delivery to damaged tissues
- Accelerated tissue remodeling under injury conditions
- Improved recovery dynamics in hypoxic environments
2. Nitric Oxide (NO) System Regulation
BPC-157 demonstrates interaction with nitric oxide signaling systems involved in:
- Vascular tone regulation
- Endothelial stability
- Blood flow modulation
- Cellular stress adaptation
These effects are believed to contribute to its observed protective properties in experimental injury models.
3. Connective Tissue Remodeling
Preclinical studies associate BPC-157 with:
- Fibroblast recruitment and migration
- Collagen synthesis organization
- Tendon and ligament healing support
- Extracellular matrix remodeling
This places BPC-157 at the center of connective tissue recovery research.
TB-500 Overview (Thymosin Beta-4 Analog)
TB-500 is a synthetic peptide modeled after naturally occurring Thymosin Beta-4, a peptide involved in cellular migration and actin regulation throughout the body.
Its biological significance is linked to:
- Cytoskeletal organization
- Cellular mobility and migration
- Tissue remodeling
- Inflammatory response modulation
- Regenerative wound-healing signaling
TB-500 is especially researched for its systemic regenerative properties, in contrast to the more localized repair signaling commonly associated with BPC-157.
TB-500 Mechanistic Research
1. Actin Binding & Cytoskeletal Dynamics
Thymosin Beta-4 is a naturally occurring actin-sequestering peptide, meaning it regulates:
- Cellular structure and movement
- Cytoskeletal remodeling
- Cell migration during tissue repair
This mechanism is fundamental in:
- Wound healing biology
- Regenerative tissue remodeling
- Structural cellular repair systems
2. Cellular Migration & Regeneration
TB-500 has been associated with:
- Keratinocyte migration
- Fibroblast movement
- Endothelial cell repair activity
- Stem-cell-associated regenerative signaling
These pathways are essential during:
- Tissue remodeling phases
- Injury recovery responses
- Structural regeneration processes
3. Inflammatory Response Modulation
Research models suggest TB-500 may influence:
- Cytokine signaling pathways
- Cellular inflammatory responses
- Oxidative stress conditions during tissue injury
This may contribute to improved regenerative efficiency in damaged tissues.
Synergistic Peptide Research Model
The scientific interest in combining BPC-157 and TB-500 stems from their potentially complementary mechanisms:
| BPC-157 | TB-500 |
|---|---|
| Vascular & localized tissue repair | Systemic cellular migration |
| Nitric oxide modulation | Cytoskeletal remodeling |
| Tendon/ligament healing pathways | Tissue-wide regenerative signaling |
| Fibroblast and collagen organization | Actin regulation and wound remodeling |
Together, the peptides are explored as a:
“Multi-pathway regenerative peptide system targeting vascular repair, connective tissue remodeling, and systemic recovery signaling.”
Research Areas
This peptide combination is commonly investigated in:
- Tendon and ligament injury models
- Muscle recovery and regeneration research
- Connective tissue remodeling studies
- Angiogenesis and vascular biology
- Wound healing pathways
- Gastrointestinal tissue integrity models
- Recovery physiology and regenerative medicine research
Scientific Summary
BPC-157 + TB-500 is best categorized as:
“A dual-regenerative peptide complex combining angiogenic repair signaling with systemic cytoskeletal remodeling and cellular migration pathways.”
Primary mechanistic domains include:
- VEGF-mediated angiogenesis
- Nitric oxide signaling modulation
- Fibroblast activation and collagen organization
- Actin regulation and cytoskeletal repair
- Cellular migration and regenerative tissue remodeling
